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Analysis of the quaternary structure of rhGPx3 mutant The purified rhGPx3 mutant was treated with loading buffer with or without β-mercaptoethanol and boiling and the resulting product was separated by SDS-PAGE
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The Cu²⁺-bound complex was characterised through the late 1970s and 1980s, with successive papers establishing the wound-healing, antioxidant and gene-regulatory effects
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