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Immunoblot analysis showed FASN protein levels were substantially decreased in a dose-dependent manner with Chac1 overexpression (Figure 5L), indicating this effect by GSH depletion is intrinsic to hepatocytes
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Measurement of free radical production using 2′,7′-dichlorofluorescein fluorescence in primary mouse hepatocytes only showed elevations after APAP metabolism, when GSH levels were depleted at 3–4 h after APAP exposure (10)
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