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CRISPR-Cas 9 transfection Cell transfection in a 6-well plate was performed using Lipofectamine CRISPRMAX (Invitrogen #: CMAX00003)

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There is the activated azygos vein pathway, left superior caval vein-azygos vein-inferior caval vein, and, consequently, congested inferior caval vein and superior mesenteric vein became decongested reflecting elimination of the otherwise severe caval and portal hypertension

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