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Abbreviations DNMT DNA methyltransferase TET Ten-eleven Translocation MBD Methyl-cytosine binding domain SAM S-adenosyl methionine CGIs CpG islands CHARM Comprehensive high-throughput arrays for relative methylation DMH Differential methylation hybridization HELP HpaII tiny fragment enrichment by ligation-mediated PCR MBD Methyl-CpG binding domain MCAM Methylated CpG island amplification microarray 5-meC 5-Methyl cytosine MeDIP Methylated DNA immunoprecipitation MIRA Methylated-CpG island recovery assay MMASS Microarray-based methylation assessment of single samples MSCC Methyl-sensitive cut counting MSO Methylation-specific oligonucleotides PMAD Promoter-associated methylated DNA amplification DNA chip RRBS Reduced representation bisulfite sequencing NGS Next-generation sequencing SNuPE Methylation-sensitive single nucleotide primer extension BSP Bisulfite sequencing PCR COBRA Combined bisulfite restriction analysis MALDI-TOF Matrix-assisted laser desorption ionization time-of-flight RLGS Restriction landmark genomic scanning LUMA Luminometric methylation assay MS-MLPA Methylation-specific multiplex ligation-dependent probe amplification MS-AFLP Methylation-sensitive amplified fragment length polymorphism Authors’ contributions SAJ and MRA designed the study and drafted the manuscript

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Diclofenac and omeprazole based validation of the Nrf2 model Since we are interested in the role of Nrf2 signaling in DILI, we set out to validate the constructed model as based on the general ROS-inducer DEM with two DILI inducing compounds